A Novel Laboratory-Based Model to Predict the Presence of Obstructive Coronary Artery Disease.

Commonly used tools to assess the probability of obstructive-coronary artery disease (CAD) were derived based on Caucasian cohorts, with their performance in China is still unknown.

Furthermore, most were established based on non-laboratory variables, contributing to the limited predictive ability to some extent. Thus, we developed and internally validated a laboratory-based model with data from a Chinese cohort of 8963 inpatients, with suspected stable chest pain, referred to catheter-based coronary angiography (CAG) from September 2007 to April 2019, and then compared the present model’s performance with the four most commonly used prediction tools, Coronary Artery Disease Consortium 1/2 Score (CAD1/2), Duke clinical score (DCS), and Diamond-Forrester score (DF).

The final model was developed by random forest method, including 8 predictors derived from 70 variables. Five-fold cross-validation was performed to evaluate the model’s prediction accuracy. In the external validation set, the present model showed a superior area under the receiver-operating curve (0.816), followed by DCS (0.66), CAD2 (0.61), CAD1 (0.59) and at last DF (0.58), respectively.

Furthermore, the present model correctly classified 74.4% of obstructive-CAD patients as high-risk, and correctly classified more than one third of non-obstructive-CAD patients as low-risk.

The present model’s net reclassification improvement (NRI) showed a significant positive reclassification over CAD1 (NRI = 0.60, P < 0.001), DF (NRI = 0.59, P < 0.001), CAD2 (NRI = 0.57, P < 0.001), and DCS (NRI = 0.43, P < 0.001). Decision curve analysis demonstrated that the present model provided a larger net benefit compared with CAD1/2, DCS, and DF.

XMIRXpress lentivector miRNA-222-3p with Xmotif
XMIRXP-222	SBI	10 ug	EUR 677
Category: Exosome

Mouse pre-microRNA Expression Construct mir-222
MMIR-222-PA-1	SBI	Bacterial Streak	EUR 684
Category: MicroRNA Tools

TA 02
B4924-10	ApexBio	10 mg	EUR 299

TA 02
B4924-5.1	ApexBio	10 mM (in 1mL DMSO)	EUR 177

TA 02
B4924-50	ApexBio	50 mg	EUR 1095

TA-02
B1215-25	Biovision		EUR 566

TA-02
B1215-5	Biovision		EUR 185

PU 02
B5687-10	ApexBio	10 mg	EUR 229

PU 02
B5687-25	ApexBio	25 mg	EUR 476

PU 02
B5687-5	ApexBio	5 mg	EUR 144

PU 02
B5687-50	ApexBio	50 mg	EUR 806

UCN-02
C4829-1	ApexBio	1 mg	EUR 460
Description: UCN-02 is a derivative of staurosporine and a stereoisomer of UCN-01, which has been first isolated from a high staurosporine-producing Streptomyces culture as a minor co-metabolite [1]. UCN-02 is protein kinase C inhibitor [1].

UCN-02
C4829-5	ApexBio	5 mg	EUR 1488
Description: UCN-02 is a derivative of staurosporine and a stereoisomer of UCN-01, which has been first isolated from a high staurosporine-producing Streptomyces culture as a minor co-metabolite [1]. UCN-02 is protein kinase C inhibitor [1].

TA-02
HY-100115	MedChemExpress	10mM/1mL	EUR 404

UCN-02
HY-108262	MedChemExpress	1mg	EUR 727

UCN-02
U006-1MG	TOKU-E	1 mg	EUR 331

UCN-02
U006-5MG	TOKU-E	5 mg	EUR 1082

OXF BD 02
B4918-10	ApexBio	10 mg	EUR 350

OXF BD 02
B4918-50	ApexBio	50 mg	EUR 1314

ELX-02 disulfate
HY-114231B	MedChemExpress	5mg	EUR 567

ELX-02 sulfate
HY-114231C	MedChemExpress	10mg	EUR 911

1961358-02-0
TBW00994	ChemNorm	10mg	Ask for price

145867-02-3
TBZ1279	ChemNorm	unit	Ask for price

Monoclonal LST1 Antibody (clone LST1/02), Clone: LST1/02
APR12482G	Leading Biology	0.05mg	EUR 484
Description: A Monoclonal antibody against Human LST1 (clone LST1/02). The antibodies are raised in Mouse and are from clone LST1/02. This antibody is applicable in WB and IHC-P, ICC, IP

VX-222 (VCH-222, Lomibuvir)
A4032-10	ApexBio	10 mg	EUR 305
Description: VX-222, a derivative of thiophene-2-carboxylic acid, is a potent non-nucleoside inhibitor (NNI) of the hepatitis C virus (HCV) RNA polymerase, an enzyme regulating RNA synthesis by a de novo-initiated mechanism or by extension from a primed template.

VX-222 (VCH-222, Lomibuvir)
A4032-100	ApexBio	100 mg	EUR 1186
Description: VX-222, a derivative of thiophene-2-carboxylic acid, is a potent non-nucleoside inhibitor (NNI) of the hepatitis C virus (HCV) RNA polymerase, an enzyme regulating RNA synthesis by a de novo-initiated mechanism or by extension from a primed template.

VX-222 (VCH-222, Lomibuvir)
A4032-5	ApexBio	5 mg	EUR 189
Description: VX-222, a derivative of thiophene-2-carboxylic acid, is a potent non-nucleoside inhibitor (NNI) of the hepatitis C virus (HCV) RNA polymerase, an enzyme regulating RNA synthesis by a de novo-initiated mechanism or by extension from a primed template.

VX-222 (VCH-222, Lomibuvir)
A4032-50	ApexBio	50 mg	EUR 838
Description: VX-222, a derivative of thiophene-2-carboxylic acid, is a potent non-nucleoside inhibitor (NNI) of the hepatitis C virus (HCV) RNA polymerase, an enzyme regulating RNA synthesis by a de novo-initiated mechanism or by extension from a primed template.

JH-VIII-157-02
HY-112140	MedChemExpress	10mM/1mL	EUR 744

Anti-07/02/07
STJ501223	St John's Laboratory	100 µg	EUR 476

VX-222
B2207-25	Biovision		EUR 675

VX-222
B2207-5	Biovision		EUR 207

VX-222
E1KS1480	EnoGene	5mg	EUR 874

QX 222
B6565-10	ApexBio	10 mg	EUR 171

QX 222
B6565-50	ApexBio	50 mg	EUR 470

VX-222
HY-75800	MedChemExpress	10mM/1mL	EUR 201

5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7*
222	AAT Bioquest	25 mg	EUR 219
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

miRZip-222 anti-miR-222 microRNA construct
MZIP222-PA-1	SBI	Bacterial Streak	EUR 684
Category: MicroRNA Tools

Monoclonal HLA-E Antibody (clone MEM-E/02), Clone: MEM-E/02
AMM01781G	Leading Biology	0.05mg	EUR 484
Description: A Monoclonal antibody against Human HLA-E (clone MEM-E/02). The antibodies are raised in Mouse and are from clone MEM-E/02. This antibody is applicable in WB and IHC-P

Monoclonal HLA-E Antibody (clone MEM-E/02), Clone: MEM-E/02
AMM02314G	Leading Biology	0.05mg	EUR 484
Description: A Monoclonal antibody against Human HLA-E (clone MEM-E/02). The antibodies are raised in Mouse and are from clone MEM-E/02. This antibody is applicable in WB and IHC-P

Septin 2 (Sep-02) Antibody
20-abx306884	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
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Septin 2 (Sep-02) Antibody (HRP)
20-abx306885	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
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Septin 2 (Sep-02) Antibody (FITC)
20-abx306886	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
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Septin 2 (Sep-02) Antibody (Biotin)
20-abx306887	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
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BTK (Ab-222) Antibody
33121-100ul	SAB	100ul	EUR 252

BTK (Ab-222) Antibody
33121-50ul	SAB	50ul	EUR 187

MAP2K3 (Ab-222) Antibody
33236-100ul	SAB	100ul	EUR 252

MAP2K3 (Ab-222) Antibody
33236-50ul	SAB	50ul	EUR 187

MAP2K3 (Ab-222) Antibody
CSB-PA084775-	Cusabio		EUR 335
Form: liquid Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific Show more
Description: A polyclonal antibody against MAP2K3 (Ab-222). Recognizes MAP2K3 (Ab-222) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:50-1:200

MAP2K3 (Ab-222) Antibody
CSB-PA084775-100ul	Cusabio	100ul	EUR 316
Form: liquid Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific Show more
Description: A polyclonal antibody against MAP2K3 (Ab-222). Recognizes MAP2K3 (Ab-222) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:50-1:200

Cytokeratin 222 Pseudogene antibody
70R-4160	Fitzgerald	50 ug	EUR 467
Description: Rabbit polyclonal Cytokeratin 222 Pseudogene antibody raised against the N terminal of KRT222P

MEK1 (pS218 / 222) Antibody
abx031877-400ul	Abbexa	400 ul	EUR 523
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MEK1 (pS218 / 222) Antibody
abx031877-80l	Abbexa	80 µl	EUR 286
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MAPK3 (pY205 / 222) Antibody
abx333481-100ul	Abbexa	100 ul	EUR 467
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rno-miR-222 Primers
MP-r00149	ABM	150 ul / 10 uM	EUR 176

hsa-miR-222 Primers
MPH01282	ABM	150 ul / 10 uM	EUR 121

mmu-miR-222 Primers
MPM00254	ABM	150 ul / 10 uM	EUR 121

Indo-1, AM *CAS 112926-02-0*
21030	AAT Bioquest	1 mg	EUR 132
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12352200

Indo-1, AM *CAS 112926-02-0*
21033	AAT Bioquest	50 mg	EUR 2181
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12352200

Z-IETD-AFC *CAS 219138-02-0*
13425	AAT Bioquest	5 mg	EUR 158
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12352200

Mouse Anti Escherichia Coli 02 Monoclonal Antibody
DMABT-51244ME	Creative Diagnostics	0.2 mg	EUR 741

Cytokeratin 222 Pseudogene Blocking Peptide
33R-2575	Fitzgerald	100 ug	EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of KRT222P antibody, catalog no. 70R-4160

Phospho-MAPK3 (Tyr205/222) Antibody
CSB-PA213371-	Cusabio		EUR 335
Form: liquid Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodie Show more
Description: A polyclonal antibody against Phospho-MAPK3 (Tyr205/222). Recognizes Phospho-MAPK3 (Tyr205/222) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

Phospho-MAPK3 (Tyr205/222) Antibody
CSB-PA213371-100ul	Cusabio	100ul	EUR 362
Form: liquid Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodie Show more
Description: A polyclonal antibody against Phospho-MAPK3 (Tyr205/222). Recognizes Phospho-MAPK3 (Tyr205/222) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

MAPK1/3 antibody (Tyr205/222)
70R-34647	Fitzgerald	100 ug	EUR 327
Description: Purified Rabbit polyclonal MAPK1/3 antibody (Tyr205/222)

MEK1/2 antibody (Ser218/222)
70R-34260	Fitzgerald	100 ug	EUR 327
Description: Rabbit polyclonal MEK1/2 antibody (Ser218/222)

MKK1 / 2 (pS218 / 222) Antibody
20-abx134438	Abbexa	EUR 314.00 EUR 467.00 EUR 203.00	100 ul 200 ul 30 ul
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BTK (Ab-222) Conjugated Antibody
C33121	SAB	100ul	EUR 397

MAP2K3 (Ab-222) Conjugated Antibody
C33236	SAB	100ul	EUR 397

Polyclonal MEK1 Antibody (S218/222)
APR06041G	Leading Biology	0.1ml	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MEK1 (S218/222). This antibody is tested and proven to work in the following applications:

MAP2K1 / MAP2K2 (pS218 / 222) Antibody
20-abx329018	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug
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Transmembrane Protein 222 (TMEM222) Antibody
20-abx302905	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
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hsa-miR-222 miRNA Inhibitor
MIH01588	ABM	2 x 5.0 nmol	EUR 176

rno-miR-222-3p Primers
MPR00529	ABM	150 ul / 10 uM	EUR 176

rno-miR-222-5p Primers
MPR00530	ABM	150 ul / 10 uM	EUR 176

hsa-miR-222-3p Primers
MPH02352	ABM	150 ul / 10 uM	EUR 121

hsa-miR-222-5p Primers
MPH02353	ABM	150 ul / 10 uM	EUR 121

mmu-miR-222 miRNA Antagomir
MNM01544	ABM	2 x 5.0 nmol	EUR 329

rno??miR-222 miRNA Antagomir
MNR01333	ABM	2 x 5.0 nmol	EUR 329

mmu-miR-222 miRNA Inhibitor
MIM01544	ABM	2 x 5.0 nmol	EUR 176

rno??miR-222 miRNA Inhibitor
MIR01333	ABM	2 x 5.0 nmol	EUR 176

mmu-miR-222-3p Primers
MPM01029	ABM	150 ul / 10 uM	EUR 121

mmu-miR-222-5p Primers
MPM01030	ABM	150 ul / 10 uM	EUR 121

hsa-miR-222 miRNA Antagomir
MNH01588	ABM	2 x 5.0 nmol	EUR 329

Anti-ZDHHC1 (aa213-222) antibody
STJ72388	St John's Laboratory	100 µg	EUR 260

MB-02 Plain plate 2mm thickness 1 set
2393036	Atto	15unit	EUR 311
Description: 2 pl at es

Tubulin-alpha (Microtubule Marker); Clone TU-02 (Concentrate)
RA0324-C.1	ScyTek Laboratories	0.1 ml	EUR 125

Tubulin-alpha (Microtubule Marker); Clone TU-02 (Concentrate)
RA0324-C.5	ScyTek Laboratories	0.5 ml	EUR 300

Recombinant P. vivax MSP1-cap [His]
DAGA-222	Creative Diagnostics	1 mg	EUR 441

HIV-1 gp120 and gp41 Chimeric Antigen (8 kDa, Capture)
DAGF-222	Creative Diagnostics	1mg	EUR 681

Rabbit Anti-ACTA1 monoclonal antibody, clone KK10-30
DCABH-222	Creative Diagnostics	100 ul	EUR 777

Recombinant Human CD93 Protein-6His-ABP tag
CTP-222	Creative Biolabs	100ug	Ask for price
Description: A 30 kDa recombinant human CD93 protein Antigen with a N-terminal His6-ABP tag expressed in E. Coli.

CytoSelect 96-Well Phagocytosis Assay (E. coli, Colorimetric Format)
CBA-222	Cell Biolabs	96 assays	EUR 693
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, E. coli Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

MAPK1/3 (Ab-205/222) Antibody
33303-100ul	SAB	100ul	EUR 252

MAPK1/3 (Ab-205/222) Antibody
33303-50ul	SAB	50ul	EUR 187

MAPKAPK-2 (Phospho-Thr221/222) Antibody
13158-100ul	SAB	100ul	EUR 252

MAPKAPK-2 (Phospho-Thr221/222) Antibody
13158-50ul	SAB	50ul	EUR 187

MAPK1/3 (Phospho-Tyr205/222) Antibody
11825-100ul	SAB	100ul	EUR 252

In conclusion, the novel model, using 8 laboratory and non-laboratory variables, performed well in risk stratifying patients with suspected chest pain regarding the presence of obstructive-CAD in the present Chinese cohort.